Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| wingless-type MMTV integration site family, member 1 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human Wnt1 (45-74aa NLLTDSKSLQLVLEPSLQLLSRKQRRLIRQ), identical to the related mouse and rat sequences. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
WNT1 |
Protein |
Proto-oncogene Wnt-1 |
Uniprot ID |
P04628 |
Function |
Ligand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters. Has a role in osteoblast function and bone development. |
Tissue Specificity |
|
Sub-cellular localization |
Secreted, extracellular space, extracellular matrix. |
Sequence Similarities |
Belongs to the Wnt family. |
Aliases |
BMND16 antibody|INT1 antibody|OI15 antibody|oncogene Int1 antibody|Proto oncogene protein Wnt 1 antibody|Proto-oncogene Int-1 homolog antibody|Proto-oncogene Wnt-1 antibody|Wingless type MMTV integration site family member 1 antibody|wingless-type MMTV integration site family, member 1 (oncogene INT1) antibody|Wnt 1 antibody|wnt1 antibody|WNT1_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- WNT1 antibody, ASA-B1987, Western blotting
All lanes: Anti WNT1 (ASA-B1987) at 0.5ug/ml
WB: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 41KD
Observed bind size: 41KD
All lanes: Anti WNT1 (ASA-B1987) at 0.5ug/ml
WB: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 41KD
Observed bind size: 41KD
