Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| high mobility group box 4 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human HMGB4(24-41aa RNKFKEQQPNTYVGFKEF), different from the related mouse and rat sequences by one amino acid. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
HMGB4 |
Protein |
High mobility group protein B4 |
Uniprot ID |
Q8WW32 |
Function |
|
Tissue Specificity |
|
Sub-cellular localization |
Nucleus. |
Sequence Similarities |
Belongs to the HMGB family. |
Aliases |
High mobility group box 4 antibody|High mobility group protein B4 antibody|HMG2 like antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-HMGB4 antibody, ASA-B0875, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Testis Tissue Lysate
Lane 3: JURKAT Cell Lysate
Lane 4: HT1080 Cell Lysate
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Testis Tissue Lysate
Lane 3: JURKAT Cell Lysate
Lane 4: HT1080 Cell Lysate
