Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| homeobox A11 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human HOXA11 recombinant protein (Position: H74-R245). Human HOXA11 shares 95% amino acid (aa) sequence identity with mouse HOXA11. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
HOXA11 |
Protein |
Homeobox protein Hox-A11 |
Uniprot ID |
P31270 |
Function |
Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. |
Tissue Specificity |
|
Sub-cellular localization |
Nucleus. |
Sequence Similarities |
Belongs to the Abd-B homeobox family. |
Aliases |
Homeo box 1I antibody|Homeo box A11 antibody|Homeobox A11 antibody|Homeobox protein Hox A11 antibody|Homeobox protein Hox-1I antibody|Homeobox protein Hox-A11 antibody|Homeobox protein HOXA11 antibody|Hox 1I antibody|Hox-1I antibody|HOX1 antibody|HOX1I antibody|HOXA11 antibody|HXA11_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- HOXA11 antibody, ASA-B0887, Western blotting
All lanes: Anti HOXA11 (ASA-B0887) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Human Placenta Tissue Lysate at 50ug
Lane 3: HLEA Whole Cell Lysate at 40ug
Lane 4: HT1080 Whole Cell Lysate at 40ug
Lane 5: HEPA Whole Cell Lysate at 40ug
Predicted bind size: 35KD
Observed bind size: 35KD
All lanes: Anti HOXA11 (ASA-B0887) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Human Placenta Tissue Lysate at 50ug
Lane 3: HLEA Whole Cell Lysate at 40ug
Lane 4: HT1080 Whole Cell Lysate at 40ug
Lane 5: HEPA Whole Cell Lysate at 40ug
Predicted bind size: 35KD
Observed bind size: 35KD
Anti- HOXA11 antibody, ASA-B0887, Western blotting
All lanes: Anti HOXA11 (ASA-B0887) at 0.5ug/ml
WB: Recombinant Human HOXA11 Protein 0.5ng
Predicted bind size: 38KD
Observed bind size: 38KD
All lanes: Anti HOXA11 (ASA-B0887) at 0.5ug/ml
WB: Recombinant Human HOXA11 Protein 0.5ng
Predicted bind size: 38KD
Observed bind size: 38KD
