Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| suppression of tumorigenicity 13(colon carcinoma)(Hsp70 interacting protein) | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human ST13(1-19aa MDPRKVNELRAFVKMCKQD), different from the related rat and mouse sequences by two amino acids. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
ST13 |
Protein |
Hsc70-interacting protein |
Uniprot ID |
P50502 |
Function |
One HIP oligomer binds the ATPase domains of at least two HSC70 molecules dependent on activation of the HSC70 ATPase by HSP40. Stabilizes the ADP state of HSC70 that has a high affinity for substrate protein. Through its own chaperone activity, it may contribute to the interaction of HSC70 with various target proteins (By similarity). |
Tissue Specificity |
|
Sub-cellular localization |
Cytoplasm . |
Sequence Similarities |
Belongs to the FAM10 family. |
Aliases |
AAG 2 antibody|AAG2 antibody|Aging associated protein 2 antibody|F10A1_HUMAN antibody|FAM10A1 antibody|FAM10A4 antibody|FLJ27260 antibody|Heat shock 70kD protein binding protein antibody|HIP antibody|HOP antibody|Hsc70 interacting protein antibody|Hsc70-interacting protein antibody|Hsp70 interacting protein antibody|HSPABP 1 antibody|HSPABP antibody|HSPABP1 antibody|MGC129952 antibody|OTTHUMP00000028873 antibody|P48 antibody|PRO0786 antibody|Progesterone receptor associated p48 protein antibody|Progesterone receptor-associated p48 protein antibody|Protein FAM10A1 antibody|Putative tumor suppressor ST13 antibody|Renal carcinoma antigen NY REN 33 antibody|Renal carcinoma antigen NY-REN-33 antibody|SNC 6 antibody|SNC6 antibody|ST 13 antibody|ST13 antibody|Suppression of tumorigenicity 13 antibody|Suppression of tumorigenicity 13(colon carcinoma) antibody|Suppression of tumorigenicity 13 protein antibody|Suppression of tumorigenicity protein 13 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-HSC70 Interacting Protein HIP antibody, ASA-B0898, Western blotting
Lane 1: A431 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: 293T Cell Lysate
Lane 4: JURKAT Cell Lysate
Lane 5: MCF-7 Cell Lysate
Lane 1: A431 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: 293T Cell Lysate
Lane 4: JURKAT Cell Lysate
Lane 5: MCF-7 Cell Lysate
