Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| heat shock 27kDa protein 2(HspB2) | Polyclonal | IgG | Rabbit | Human, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human HSPB2 recombinant protein (Position: M1-P182). Human HSPB2 shares 97.3% amino acid (aa) sequence identity with both mouse and rat HSPB2. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
HSPB2 |
Protein |
Heat shock protein beta-2 |
Uniprot ID |
Q16082 |
Function |
May regulate the kinase DMPK. |
Tissue Specificity |
Expressed preferentially in skeletal muscle and heart but not in the lens. |
Sub-cellular localization |
Cytoplasm. |
Sequence Similarities |
Belongs to the small heat shock protein (HSP20) family. |
Aliases |
DMPK-binding protein antibody|Heat shock 27kD protein 2 antibody|Heat shock 27kDa protein 2 antibody|Heat shock protein beta-2 antibody| Heat-shock protein beta-2 antibody|Hs.78846 antibody|HSP27 antibody|HspB2 antibody|HSPB2_HUMAN antibody|LOH11CR1K antibody| MGC133245 antibody|MKBP antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat Human | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-HSPB2 antibody, ASA-B0929, Western blotting
All lanes: Anti HSPB2 (ASA-B0929) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 2: Rat Skeletal Muscle Tissue Lysate at 50ug
Predicted bind size: 20KD
Observed bind size: 20KD
All lanes: Anti HSPB2 (ASA-B0929) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 2: Rat Skeletal Muscle Tissue Lysate at 50ug
Predicted bind size: 20KD
Observed bind size: 20KD
