Description
Overview
Long Name
|
Antibody Type
|
Antibody Isotype
|
Host
|
Species Reactivity
|
Validated Applications
|
Purification
|
| B-cell CLL/lymphoma 2 |
Polyclonal |
IgG |
Rabbit |
Human |
ELISA, Neu, IP, WB |
Immunogen affinity purified. |
Immunogen
|
| E. coli-derived human BCL-2 recombinant protein(Position: M1-D211). |
Properties
Form
|
Lyophilized |
Size
|
100 µg/vial |
Contents
|
Antibody is lyophilized with 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration
|
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage
|
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene
|
BCL2 |
Protein
|
Apoptosis regulator Bcl-2 |
Uniprot ID
|
P10415 |
Function
|
Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). |
Tissue Specificity
|
Expressed in a variety of tissues. |
Sub-cellular localization
|
Mitochondrion outer membrane. |
Sequence Similarities
|
Belongs to the Bcl-2 family. |
Aliases
|
Apoptosis regulator Bcl2 antibody|B cell CLL antibody|B cell lymphoma 2 antibody|B cell lymphoma protein 2 alpha isoform antibody|B cell lymphoma protein 2 antibody|B-cell CLL/lymphoma 2 antibody|B-cell leukemia/lymphoma 2 antibody|Bcl 2 antibody|bcl2a antibody|Leukemia /lymphoma, B-cell, 2 antibody|Oncogene B cell leukemia 2 antibody|Oncogene B-cell leukemia 2 antibody |
Application Details
| Application |
Concentration* |
Species |
Validated Using** |
| Western blot |
0.1-0.5μg/ml |
Human |
AssaySolutio’s ECL kit |
| ELISA |
0.1-0.5μg/ml |
Human |
Sandwich ELISA format |
| Neutralization |
Human |
Human |
|
| Immunoprecipitation |
Human |
Human |
|
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
