Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| interleukin 1, beta | Polyclonal | IgG | Rabbit | Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of mouse IL1 beta(250-265aa FLGNNSGQDIIDFTME), different from the related rat sequence by four amino acids. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
IL1B |
Protein |
Interleukin-1 beta(IL-1 beta) |
Uniprot ID |
P10749 |
Function |
Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. |
Tissue Specificity |
|
Sub-cellular localization |
Secreted. Note: The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. |
Sequence Similarities |
|
Aliases |
Catabolin antibody|H1 antibody|Hematopoietin 1 antibody|IFN beta inducing factor antibody|IL 1 antibody|IL 1 beta antibody|IL 1B antibody|IL-1 beta antibody|IL1 BETA antibody|IL1B antibody|IL1B_HUMAN antibody|IL1F2 antibody|Interleukin 1 beta antibody|Interleukin 1 beta precursor antibody|Interleukin-1 beta antibody|LAF antibody|OAF antibody|Osteoclast activating factor antibody|OTTHUMP00000162031 antibody|Preinterleukin 1 beta antibody|Preinterleukin beta antibody|Pro interleukin 1 beta antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse, Rat | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-IL1 beta antibody, ASA-B0996, Western blotting
Lane 1: Recombinant Mouse IL1 beta Protein 10ng
Lane 2: Recombinant Mouse IL1 beta Protein 5ng
Lane 3: Recombinant Mouse IL1 beta Protein 2.5ng
Lane 1: Recombinant Mouse IL1 beta Protein 10ng
Lane 2: Recombinant Mouse IL1 beta Protein 5ng
Lane 3: Recombinant Mouse IL1 beta Protein 2.5ng
