Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| interleukin 12B(natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human IL12 p40(23-40aa IWELKKDVYVVELDWYPD). | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
IL12B |
Protein |
Interleukin-12 subunit beta(IL-12B) |
Uniprot ID |
P29460 |
Function |
Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine- activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. |
Tissue Specificity |
|
Sub-cellular localization |
Secreted. |
Sequence Similarities |
Belongs to the type I cytokine receptor family. Type 3 subfamily. |
Aliases |
40 kD Subunit antibody|CLMF 2 antibody|CLMF antibody|CLMF p40 antibody|CLMF2 antibody|Cytotoxic lymphocyte maturation factor 2 antibody|Cytotoxic lymphocyte maturation factor 2 p40 antibody|Cytotoxic lymphocyte maturation factor 40 kDa subunit antibody|HGNC:5970 antibody|IL 12 subunit p40 antibody|IL 12B antibody|IL-12 subunit p40 antibody|IL-12B antibody|IL12 subunit p40 antibody|IL12B antibody|IL12B_HUMAN antibody|Interleukin 12 beta chain antibody|Interleukin 12 p40 antibody|Interleukin 12 subunit beta antibody|Interleukin 12B antibody|Interleukin-12 subunit beta antibody|Natural killer cell stimulatory factor 2 antibody|NK cell stimulatory factor chain 2 antibody|NKSF 2 antibody|NKSF antibody|NKSF2 antibody|p40 antibody|TcMF antibody|TSF antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-IL12 p40 antibody, ASA-B1000, Western blotting
Lane 1: Recombinant Human IL-12 Protein 10ng
Lane 2: Recombinant Human IL-12 Protein 5ng
Lane 3: Recombinant Human IL-12 Protein 2.5ng
Lane 1: Recombinant Human IL-12 Protein 10ng
Lane 2: Recombinant Human IL-12 Protein 5ng
Lane 3: Recombinant Human IL-12 Protein 2.5ng
