Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| interleukin 2 | Polyclonal | IgG | Rabbit | Mouse | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of mouse IL2(151-169aa DFLRRWIAFCQSIISTSPQ). | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
IL2 |
Protein |
Interleukin-2(IL-2) |
Uniprot ID |
P04351 |
Function |
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine- activated killer cells, natural killer cells, and glioma cells. |
Tissue Specificity |
|
Sub-cellular localization |
Secreted. |
Sequence Similarities |
|
Aliases |
Aldesleukin antibody|IL 2 antibody|IL-2 antibody|IL2 antibody|IL2_HUMAN antibody|Interleukin 2 antibody|Interleukin-2 antibody|Interleukin2 antibody|Involved in regulation of T cell clonal expansion antibody|Lymphokine antibody|OTTHUMP00000164090 antibody|POIL2 antibody|T-cell growth factor antibody|TCGF antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-IL2 antibody, ASA-B1012, Western blotting
Lane 1: Recombinant Mouse IL2 Protein 10ng
Lane 2: Recombinant Mouse IL2 Protein 5ng
Lane 3: Recombinant Mouse IL2 Protein 2.5ng
Lane 1: Recombinant Mouse IL2 Protein 10ng
Lane 2: Recombinant Mouse IL2 Protein 5ng
Lane 3: Recombinant Mouse IL2 Protein 2.5ng
