Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| interleukin 3 | Polyclonal | IgG | Rabbit | Rat | ELISA, WB | Immunogen affinity purified. |
Immunogen |
||||||
| E. coli-derived rat IL3 recombinant protein (Position: S28-C166). Rat IL3 shares 32% and 55% amino acid (aa) sequence identity with human and mouse IL3, respectively. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
IL3 |
Protein |
Interleukin-3 |
Uniprot ID |
P04823 |
Function |
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. |
Tissue Specificity |
Activated T-cells, mast cells, natural killer cells. |
Sub-cellular localization |
Secreted. |
Sequence Similarities |
|
Aliases |
Colony stimulating factor multiple antibody|Hematopoietic growth factor antibody|IL 3 antibody|IL-3 antibody|IL3 antibody|IL3_HUMAN antibody|Interleukin 3 (colony stimulating factor, multiple) antibody|Interleukin 3 antibody|Interleukin-3 antibody|Mast cell growth factor antibody|MCGF antibody|MGC79398 antibody|MGC79399 antibody|Multi CSF antibody|Multilineage colony stimulating factor antibody|Multipotential colony stimulating factor antibody|Multipotential colony-stimulating factor antibody|OTTHUMP00000065963 antibody|P cell stimulating factor antibody|P-cell-stimulating factor antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat | AssaySolutio’s ECL kit |
| ELISA | 0.1-0.5μg/ml | Rat | Sandwich ELISA format |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- IL3 antibody, ASA-B1021, Western blotting
All lanes: Anti IL3 (ASA-B1021) at 0.5ug/ml
WB: Recombinant Rat IL3 Protein 0.5ng
Predicted bind size: 15KD
Observed bind size: 15KD
All lanes: Anti IL3 (ASA-B1021) at 0.5ug/ml
WB: Recombinant Rat IL3 Protein 0.5ng
Predicted bind size: 15KD
Observed bind size: 15KD
Anti- IL3 antibody, ASA-B1021, Western blotting
All lanes: Anti IL3 (ASA-B1021) at 0.5ug/ml
Lane 1: PC-12 Whole Cell Lysate at 40ug
Lane 2: NRK Whole Cell Lysate at 40ug
Lane 3: RH35 Whole Cell Lysate at 40ug
Predicted bind size: 17KD
Observed bind size: 37KD
All lanes: Anti IL3 (ASA-B1021) at 0.5ug/ml
Lane 1: PC-12 Whole Cell Lysate at 40ug
Lane 2: NRK Whole Cell Lysate at 40ug
Lane 3: RH35 Whole Cell Lysate at 40ug
Predicted bind size: 17KD
Observed bind size: 37KD
