Description
Overview
Long Name
|
Antibody Type
|
Antibody Isotype
|
Host
|
Species Reactivity
|
Validated Applications
|
Purification
|
| actin, alpha 2, smooth muscle, aorta |
Monoclonal |
IgG |
Mouse |
Human, Mouse, Rat |
IHC-P, IHC-F, WB |
Ascites |
Immunogen
|
| N-terminal synthetic decapeptide of alpha-smooth muscle actin. |
Properties
Form
|
Lyophilized |
Size
|
100 µg/vial |
Contents
|
Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration
|
1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage
|
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene
|
1867 |
Protein
|
Actin, aortic smooth muscle |
Uniprot ID
|
Human(P62736), Mouse(P62737), Rat(P62738) |
Function
|
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. |
Tissue Specificity
|
|
Sub-cellular localization
|
Cytoplasm, cytoskeleton. |
Sequence Similarities
|
|
Aliases
|
AAT6|ACTSA|MYMY5|ACTSA|alpha-cardiac actin|cell growth-inhibiting gene 46 protein |
Application Details
| Application |
Concentration* |
Species |
Validated Using** |
| Western blot |
2μg/ml |
Human, mouse, rat. |
AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) |
0.1-0.5μg/ml |
Human, mouse, rat. |
AssaySolutio’s IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) |
0.1-0.5μg/ml |
Human, mouse, rat. |
AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-alpha-Smooth Muscle Actin antibody (monoclonal), ASA-B0077, IHC(P)
Rat Cardiac Muscle Tissue
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