Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| apoptotic peptidase activating factor 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human APAF1(1-19 MDAKARNCLLQHREALEKD), identical to the related mouse sequence, and different from the related rat sequence by one amino acid. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
APAF1 |
Protein |
Apoptotic protease-activating factor 1(APAF-1) |
Uniprot ID |
O14727 |
Function |
Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. |
Tissue Specificity |
Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver. |
Sub-cellular localization |
Cytoplasm. |
Sequence Similarities |
Contains 1 CARD domain. |
Aliases |
APAF 1 antibody|Apaf-1 antibody|APAF_HUMAN antibody|Apaf1 antibody|Apoptotic peptidase activating factor 1 antibody|Apoptotic protease activating factor 1 antibody|Apoptotic protease activating factor antibody|Apoptotic protease-activating factor 1 antibody|CED 4 antibody|CED4 antibody|KIAA0413 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Rat Human, Mouse | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- APAF1antibody, ASA-B0108, Western blotting
All lanes: Anti APAF1 (ASA-B0108) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: COLO320 Whole Cell Lysate at 40ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Predicted band size: 140KD
Observed band size: 140KD
All lanes: Anti APAF1 (ASA-B0108) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: COLO320 Whole Cell Lysate at 40ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Predicted band size: 140KD
Observed band size: 140KD
