Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| aquaporin 2 (collecting duct) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 2 (241-271aa EPDTDWE- EREVRRRQSVELHSPQSLPRGTKA), different from the related mouse and rat sequences by one amino acid. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
AQP2 |
Protein |
Aquaporin-2 |
Uniprot ID |
P41181 |
Function |
Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. |
Tissue Specificity |
Expressed in renal collecting tubules. |
Sub-cellular localization |
Apical cell membrane. |
Sequence Similarities |
Belongs to the MIP/aquaporin (TC 1.A.8) family. |
Aliases |
ADH water channel antibody|AQP 2 antibody|AQP CD antibody|AQP-2 antibody| AQP-CD antibody| AQP2 antibody|AQP2_HUMAN antibody|AQPCD antibody| Aquaporin 2 collecting duct antibody| Aquaporin CD antibody|Aquaporin-2 antibody|Aquaporin-CD antibody|Aquaporin2 antibody| Aquaporine 2 antibody| Collecting duct water channel protein antibody|MGC34501 antibody| Water channel aquaporin 2 antibody|Water channel protein for renal collecting duct antibody|WCH CD antibody|WCH-CD antibody|WCHCD antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Aquaporin 2 antibody, ASA-B0136, Western blotting
All lanes: Anti Aquaporin 2 (ASA-B0136) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 50ug
Lane 2: Mouse Kidney Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: A549 Whole Cell Lysate at 40ug
Lane 5: PANC Whole Cell Lysate at 40ug
Predicted band size: 29KD
Observed aind size: 50KD
All lanes: Anti Aquaporin 2 (ASA-B0136) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 50ug
Lane 2: Mouse Kidney Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: A549 Whole Cell Lysate at 40ug
Lane 5: PANC Whole Cell Lysate at 40ug
Predicted band size: 29KD
Observed aind size: 50KD
Anti- Aquaporin 2 antibody, ASA-B0136, IHC(P)
Mouse Kidney Tissue
Mouse Kidney Tissue
Anti- Aquaporin 2 antibody, ASA-B0136, IHC(P)
Rat Kidney Tissue
Rat Kidney Tissue
