Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| ATPase, Cu++ transporting, beta polypeptide | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human ATP7b (1451-1465aa, DKWSLLLNGRDEEQYI), different from the related mouse and rat sequences by three amino acids. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
ATP7B |
Protein |
Copper-transporting ATPase 2 |
Uniprot ID |
P35670 |
Function |
Involved in the export of copper out of the cells, such as the efflux of hepatic copper into the bile. |
Tissue Specificity |
Most abundant in liver and kidney and also found in brain. Isoform 2 is expressed in brain but not in liver. The cleaved form WND/140 kDa is found in liver cell lines and other tissues. |
Sub-cellular localization |
Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. Note: Predominantly found in the trans-Golgi network (TGN). Not redistributed to the plasma membrane in response to elevated copper levels. |
Sequence Similarities |
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily. |
Aliases |
ATP7B antibody|ATPase Cu++ transporting beta polypeptide antibody|ATPase Cu++ transporting beta polypeptide antibody|ATPase, Cu(2+) transporting, beta polypeptide antibody|ATPase, Cu++ transporting, beta polypeptide antibody|Copper pump 2 antibody|Copper transporting ATPase 2 antibody|PWD antibody|Toxic milk antibody|tx antibody|WC1 antibody|WD antibody|Wilson disease associated protein antibody|Wilson disease-associated protein antibody|WND antibody|WND/140 kDa antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-ATP7b antibody, ASA-B0176, IHC(P)
Mouse Liver Tissue
Mouse Liver Tissue
Anti-ATP7b antibody, ASA-B0176, IHC(P)
Rat Liver Tissue
Rat Liver Tissue
Anti-ATP7b antibody, ASA-B0176, IHC(P)
Rat Kidney Tissue
Rat Kidney Tissue
Anti-ATP7b antibody, ASA-B0176, Western blot
All lanes: Anti-ATP7B(PA2262) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 40ug
Lane 2: Rat Brain Tissue Lysate at 40ug
Lane 3: SMMC Whole Cell Lysate at 40ug
Lane 4: NEURO Whole Cell Lysate at 40ug
Predicted band size: 157KD
Observed band size: 157KD
Lane 1: Rat Liver Tissue Lysate at 40ug
Lane 2: Rat Brain Tissue Lysate at 40ug
Lane 3: SMMC Whole Cell Lysate at 40ug
Lane 4: NEURO Whole Cell Lysate at 40ug
Predicted band size: 157KD
Observed band size: 157KD
