Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| BCL2-associated athanogene 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human BAG2 recombinant protein (Position: M1-N211). Human BAG2 shares 93.4% amino acid (aa) sequence identity with mouse BAG2. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
BAG2 |
Protein |
BAG family molecular chaperone regulator 2 |
Uniprot ID |
O95816 |
Function |
Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. |
Tissue Specificity |
|
Sub-cellular localization |
|
Sequence Similarities |
|
Aliases |
BAG 2 antibody|BAG family molecular chaperone regulator 2 antibody|BAG-2 antibody|BAG-family molecular chaperone regulator-2 antibody|Bag2 antibody| BAG2_HUMAN antibody|BCL 2 associated athanogene 2 antibody|Bcl-2-associated athanogene 2 antibody|BCL2 associated athanogene 2 antibody| BCL2-associated athanogene 2 antibody|dJ417I1.2 (BAG family molecular chaperone regulator 2) antibody|dJ417I1.2 antibody|KIAA0576 antibody| MGC149462 antibody| OTTHUMP00000016668 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- BAG2 antibody, ASA-B0190, Western blotting
All lanes: Anti BAG2 (ASA-B0190) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Predicted band size: 24KD
Observed band size: 24KD
All lanes: Anti BAG2 (ASA-B0190) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Predicted band size: 24KD
Observed band size: 24KD
Anti- BAG2 antibody, ASA-B0190,IHC(P)
Mouse Testis Tissue
Mouse Testis Tissue
Anti- BAG2 antibody, ASA-B0190,IHC(P)
Rat Testis Tissue
Rat Testis Tissue
Anti- BAG2 antibody, ASA-B0190,IHC(P)
Human Pancreatic Cancer Tissue
