Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| budding uninhibited by benzimidazoles 3 homolog(yeast) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, IHC-F, ICC, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence in the middle region of human Bub3(76-90aa DHQLKMHDLNTDQEN), identical to the related rat and mouse sequences. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
BUB3 |
Protein |
Mitotic checkpoint protein BUB3 |
Uniprot ID |
O43684 |
Function |
Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. |
Tissue Specificity |
|
Sub-cellular localization |
Nucleus. Chromosome, centromere, kinetochore . Note: Starts to localize at kinetochores in prometaphase I (Pro-MI) stage and maintains the localization until the metaphase I-anaphase I (MI-AI) transition. |
Sequence Similarities |
Belongs to the WD repeat BUB3 family. |
Aliases |
Aa2-050 antibody|AU019800 antibody|AU021329 antibody|AU043350 antibody|AW146323 antibody|BUB 3 antibody|BUB 3L antibody|BUB3(budding uninhibited by benzimidazoles 3, yeast) homolog antibody|bub3 antibody|BUB3 budding uninhibited by benzimidazoles 3 homolog antibody|BUB3_HUMAN antibody|BUB3L antibody|Budding uninhibited by benomyl antibody|Budding uninhibited by benzimidazoles 3 antibody|Budding uninhibited by benzimidazoles 3 homolog(S. cerevisiae) antibody|Budding uninhibited by benzimidazoles 3 homolog(yeast) antibody|Budding uninhibited by benzimidazoles 3 homolog antibody|Budding uninhibited by benzimidazoles 3, S. cerevisiae, homolog of antibody|C78067 antibody|hBUB3 antibody|Mitotic checkpoint component antibody|Mitotic checkpoint protein bub3 antibody|OTTHUMP00000020679 antibody|OTTHUMP00000020680 antibody|WD repeat type I transmembrane protein A72.5 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio’s IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Rat Human, Mouse | AssaySolutio’s IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Bub3 antibody, ASA-B0246, Western blotting
All lanes: Anti Bub3 (ASA-B0246) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 37KD
Observed bind size: 37KD
All lanes: Anti Bub3 (ASA-B0246) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 37KD
Observed bind size: 37KD
Anti-Bub3 antibody, ASA-B0246, IHC(P)
IHC(P): Rat Testis Tissue
IHC(P): Rat Testis Tissue
Anti-Bub3 antibody, ASA-B0246, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
