Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| calcium/calmodulin-dependent protein kinase kinase 1, alpha | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of mouse CaMKK(10-31aa QDPRAELVDRVAAINVAHLEEA), different from the related human sequence by three amino acids, and from the related rat sequence by two amino acids. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
CAMKK1 |
Protein |
Calcium/calmodulin-dependent protein kinase kinase 1(CaM-KK 1/CaM-kinase kinase 1/CaMKK 1) |
Uniprot ID |
Q8VBY2 |
Function |
Calcium/calmodulin-dependent protein kinase that belongs to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Phosphorylates CAMK1, CAMK1D, CAMK1G and CAMK4. Involved in regulating cell apoptosis. Promotes cell survival by phosphorylating AKT1/PKB that inhibits pro-apoptotic BAD/Bcl2-antagonist of cell death. |
Tissue Specificity |
Widely expressed. Differentially expressed in various brain regions. |
Sub-cellular localization |
Cytoplasm . Nucleus . |
Sequence Similarities |
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. |
Aliases |
Ca2+/calmodulin-dependent protein kinase kinase alpha antibody|Calcium/calmodulin dependent protein kinase kinase antibody|Calcium/calmodulin dependent protein kinase kinase 1 alpha antibody|Calcium/calmodulin dependent protein kinase kinase 1 antibody|Calcium/calmodulin dependent protein kinase kinase alpha antibody|Calcium/calmodulin-dependent protein kinase kinase 1 antibody|Calcium/calmodulin-dependent protein kinase kinase alpha antibody|CaM kinase kinase alpha antibody|CaM-kinase IV kinase antibody|CaM-kinase kinase 1 antibody|CaM-kinase kinase alpha antibody|CaM-KK 1 antibody|CaM-KK alpha antibody|CaMKK 1 antibody|CaMKK alpha antibody|CAMKK alpha protein antibody|CaMKK antibody|CAMKK1 antibody|CAMKKA antibody|DKFZp761M0423 antibody|KKCC1_HUMAN antibody|MGC34095 antibody|OTTMUSP00000006419 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Rat Mouse | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CaMKK antibody, ASA-B0275, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Mouse Brain Tissue Lysate
Lane 4: Mouse Brain Tissue Lysate
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Mouse Brain Tissue Lysate
Lane 4: Mouse Brain Tissue Lysate
Anti-CaMKK antibody, ASA-B0275, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue
