Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| carbonic anhydrase III, muscle specific | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human Carbonic Anhydrase III(2-20aa AKEWGYASHNGPDHWHELF), different from the related mouse and rat sequence by two amino acids. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
CA3 |
Protein |
Carbonic anhydrase 3 |
Uniprot ID |
P07451 |
Function |
Reversible hydration of carbon dioxide. |
Tissue Specificity |
Muscle specific. |
Sub-cellular localization |
Cytoplasm. |
Sequence Similarities |
|
Aliases |
CA III antibody|CA-III antibody|Ca3 antibody|CAH3_HUMAN antibody|CAIII antibody|Car3 antibody|Carbonate dehydratase III antibody|Carbonic anhydrase 3 antibody|Carbonic anhydrase III antibody|Carbonic anhydrase III muscle specific antibody|EC 4.2.1.1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Rat Mouse | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Carbonic Anhydrase III antibody, ASA-B0278, Western blotting
Lane 1: SMMC Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: SW620 Cell Lysate
Lane 4: SCG Cell Lysate
Lane 1: SMMC Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: SW620 Cell Lysate
Lane 4: SCG Cell Lysate
Anti-Carbonic Anhydrase III antibody, ASA-B0278, IHC(P)
IHC(P): Rat Skeletal Muscle Tissue
IHC(P): Rat Skeletal Muscle Tissue
