Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| caspase 4, apoptosis-related cysteine peptidase | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human Caspase 4(104-124aa DALKLCPHEEFLRLCKERAEE). | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
CASP4 |
Protein |
Caspase-4 |
Uniprot ID |
P49662 |
Function |
Involved in the activation cascade of caspases responsible for apoptosis execution. Involved in ER-stress induced apoptosis. Cleaves caspase-1. |
Tissue Specificity |
Widely expressed, with highest levels in spleen and lung. Moderate expression in heart and liver, low expression in skeletal muscle, kidney and testis. Not found in the brain. |
Sub-cellular localization |
Endoplasmic reticulum membrane. Mitochondrion. |
Sequence Similarities |
Belongs to the peptidase C14A family. |
Aliases |
Apoptosis related cysteine peptidase antibody|Apoptotic cysteine protease Mih1/TX antibody|CASP 4 antibody|CASP 4 antibody|CASP-4 antibody|CASP4 antibody|CASP4_HUMAN antibody|Caspase 4 apoptosis related cysteine peptidase antibody|Caspase-4 subunit 2 antibody|Caspase4 antibody|ICE(rel) II antibody|ICE(rel)-II antibody|ICE(rel)II antibody|ICEREL II antibody|ICERELII antibody|ICH 2 antibody|ICH 2 protease antibody|ICH2 antibody|Mih1/TX antibody|Mih1/TX antibody|Protease ICH-2 antibody|Protease TX antibody|TX antibody|TX protease antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Caspase 4 antibody, ASA-B0297, Western blotting
All lanes: Anti Caspase 4 (ASA-B0297) at 0.5ug/ml
Lane 1: MCF-7 Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Lane 4: CEM Whole Cell Lysate at 40ug
Lane 5: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 43KD
Observed bind size: 20KD
All lanes: Anti Caspase 4 (ASA-B0297) at 0.5ug/ml
Lane 1: MCF-7 Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Lane 4: CEM Whole Cell Lysate at 40ug
Lane 5: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 43KD
Observed bind size: 20KD
Anti-Caspase 4 antibody, ASA-B0297, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
