Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| cathepsin D | Polyclonal | IgG | Rabbit | Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived mouse Cathepsin D recombinant protein (Position: E65-L410). Mouse Cathepsin D shares 85% and 91% amino acid (aa) sequences identity with human and rat Cathepsin D, respectively. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
CTSD |
Protein |
Cathepsin D |
Uniprot ID |
P18242 |
Function |
Acid protease active in intracellular protein breakdown. |
Tissue Specificity |
|
Sub-cellular localization |
Lysosome. Melanosome . Secreted, extracellular space . |
Sequence Similarities |
|
Aliases |
CatD antibody|CATD_HUMAN antibody|Cathepsin D heavy chain antibody|Cathepsin D light chain antibody|CathepsinD antibody|CD antibody|Ceroid lipofuscinosis neuronal 10 antibody|CLN10 antibody|CPSD antibody|CTSD antibody|Lysosomal aspartyl peptidase antibody|Lysosomal aspartyl protease antibody|MGC2311 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Mouse, Rat | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Cathepsin D antibody, ASA-B0313–1.jpg
IHC(P): Mouse Kidney Tissue
IHC(P): Mouse Kidney Tissue
Anti-Cathepsin D antibody, ASA-B0313–2.jpg
IHC(P): Mouse Liver Tissue
IHC(P): Mouse Liver Tissue
Anti-Cathepsin D antibody, ASA-B0313–3.jpg
All lanes: Anti-Cathepsin D(ASA-B0313) at 0.5ug/ml
Lane 1: Mouse Liver Tissue Lysate at 40ug
Lane 2: Mouse Brain Tissue Lysate at 40ug
Lane 3: Mouse Thymus Tissue Lysate at 40ug
Lane 4: NEURO Whole Cell Lysate at 40ug
Predicted bind size: 45KD
Observed bind size: 45KD
All lanes: Anti-Cathepsin D(ASA-B0313) at 0.5ug/ml
Lane 1: Mouse Liver Tissue Lysate at 40ug
Lane 2: Mouse Brain Tissue Lysate at 40ug
Lane 3: Mouse Thymus Tissue Lysate at 40ug
Lane 4: NEURO Whole Cell Lysate at 40ug
Predicted bind size: 45KD
Observed bind size: 45KD
