Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| CD46 molecule, complement regulatory protein | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human CD46 (366-392aa YRYLQRRKKKGTYLTDETHREVKFTSL). | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
CD46 |
Protein |
Membrane cofactor protein |
Uniprot ID |
P15529 |
Function |
Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement- mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T- cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity. A number of viral and bacterial pathogens seem to exploit this property and directly induce an immunosuppressive phenotype in T-cells by binding to CD46. |
Tissue Specificity |
Expressed by all cells except erythrocytes. |
Sub-cellular localization |
Cytoplasmic vesicle, secretory vesicle, acrosome inner membrane. |
Sequence Similarities |
Contains 4 Sushi (CCP/SCR) domains. |
Aliases |
AHUS2 antibody|Antigen defined by monoclonal antibody| TRA 2 10 antibody| Antigen identified by monoclonal antibody| TRA 2 10 antibody|CD46 antibody| CD46 antigen antibody|CD46 antigen complement regulatory protein antibody| CD46 molecule antibody|CD46 molecule complement regulatory protein antibody|Complement membrane cofactor protein antibody|MCP antibody| MCP_HUMAN antibody|Measles virus receptor antibody|membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen) antibody| Membrane cofactor protein antibody| MGC26544 antibody|MIC10 antibody|TLX antibody|TRA2.10 antibody|Trophoblast leucocyte common antigen antibody| Trophoblast leukocyte common antigen antibody|Trophoblast lymphocyte cross reactive antigen antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- CD46 antibody, ASA-B0388, Western blotting
All lanes: Anti CD46 (ASA-B0388) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 49KD
Observed bind size: 49KD
All lanes: Anti CD46 (ASA-B0388) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 49KD
Observed bind size: 49KD
Anti- CD46 antibody, ASA-B0388, IHC(P)
IHC(P): Human Prostatic Cancer Tissue
IHC(P): Human Prostatic Cancer Tissue
