Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human c-Kit recombinant protein (Position: Q26-S285). Human c-Kit shares 66% amino acid (aa) sequence identity with mouse c-Kit. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
KIT |
Protein |
Mast/stem cell growth factor receptor Kit |
Uniprot ID |
P10721 |
Function |
Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1. |
Tissue Specificity |
Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells. |
Sub-cellular localization |
Isoform 1: Cell membrane; Single-pass type I membrane protein. |
Sequence Similarities |
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. |
Aliases |
C Kit antibody|c-Kit antibody|CD 117 antibody|CD117 antibody|CD117 antigen antibody|KIT antibody|KIT oncogene antibody|KIT_HUMAN antibody|Mast cell growth factor receptor antibody|Mast/stem cell growth factor receptor antibody|Mast/stem cell growth factor receptor Kit antibody|p145 c-kit antibody|PBT antibody|Piebald trait protein antibody|Proto oncogene c Kit antibody|Proto oncogene tyrosine protein kinase Kit antibody|Proto-oncogene c-Kit antibody|SCF Receptor antibody|SCFR antibody|soluble KIT variant 1 antibody|Steel Factor Receptor antibody|Stem cell factor receptor antibody|tyrosine protein kinase Kit antibody|Tyrosine-protein kinase Kit antibody|v kit Hardy Zuckerman 4 feline sarcoma viral oncogene homolog antibody|v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein antibody|v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- C-Kit antibody, ASA-B0249, Western blotting
All lanes: Anti C-Kit (ASA-B0249) at 0.5ug/ml
WB: Recombinan Human C-Kit Protein 0.5ng
Predicted bind size: 49KD
Observed bind size: 49KD
All lanes: Anti C-Kit (ASA-B0249) at 0.5ug/ml
WB: Recombinan Human C-Kit Protein 0.5ng
Predicted bind size: 49KD
Observed bind size: 49KD
Anti- C-Kit antibody, ASA-B0249, Western blotting
All lanes: Anti C-Kit (ASA-B0249) at 0.5ug/ml
WB: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 109KD
Observed bind size: 109KD
All lanes: Anti C-Kit (ASA-B0249) at 0.5ug/ml
WB: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 109KD
Observed bind size: 109KD
Anti- C-Kit antibody, ASA-B0249, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue
