Description
Overview
Long Name
|
Antibody Type
|
Antibody Isotype
|
Host
|
Species Reactivity
|
Validated Applications
|
Purification
|
| v-myc myelocytomatosis viral oncogene homolog(avian) |
Monoclonal |
IgG |
Mouse |
Human |
IHC-P, ICC, WB |
Ascites |
Immunogen
|
| Synthetic peptide corresponding to residues 408-439 of the human p62c-Myc protein. |
Properties
Form
|
Lyophilized |
Size
|
100 µg/vial |
Contents
|
Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration
|
1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage
|
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene
|
MYC |
Protein
|
Myc proto-oncogene protein |
Uniprot ID
|
Human(P01106), Mouse(P01108), Rat(P09416) |
Function
|
Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5′- CAC[GA]TG-3′. Activates the transcription of growth-related genes. |
Tissue Specificity
|
|
Sub-cellular localization
|
Nucleus, nucleoplasm. |
Sequence Similarities
|
Contains 1 bHLH (basic helix-loop-helix) domain. |
Aliases
|
C-Myc|bHLHe39|Proto-oncogene c-Myc|Transcription factor p64|Class E basic helix-loop-helix protein 39 |
Application Details
| Application |
Concentration* |
Species |
Validated Using** |
| Western blot |
4μg/ml |
Human |
AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) |
8μg/ml |
Human |
AssaySolutio’s IHC/ICC Detection kit |
| Immunocytochemistry |
Suitable |
Human |
AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-c-Myc antibody (monoclonal), ASA-B0251, IHC(P)
IHC(P): Rat Cardiac Muscle Tissue