Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| ciliary neurotrophic factor | Polyclonal | IgG | Rabbit | Mouse, Rat | ELISA, IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| E. coli-derived rat CNTF recombinant protein (Position: A2-M200). Rat CNTF shares 84% and 95% amino acid (aa) sequences identity with human and mouse CNTF, respectively. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
CNTF |
Protein |
Ciliary neurotrophic factor |
Uniprot ID |
P20294 |
Function |
CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. |
Tissue Specificity |
Nervous system. |
Sub-cellular localization |
Cytoplasm. |
Sequence Similarities |
|
Aliases |
Ciliary Neurotrophic Factor antibody|CNTF antibody|CNTF_HUMAN antibody|HCNTF antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Mouse, Rat | AssaySolutio’s IHC/ICC Detection kit |
| ELISA | 0.1-0.5μg/ml | Rat | Sandwich ELISA format |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CNTF antibody, ASA-B0465–1.jpg
IHC(P): Mouse Brain Tissue
IHC(P): Mouse Brain Tissue
Anti-CNTF antibody, ASA-B0465–2.jpg
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue
Anti-CNTF antibody, ASA-B0465–3.jpg
All lanes: Anti-CNTF(ASA-B0465) at 0.5ug/ml
Lane 1: PC-12 Whole Cell Lysate at 40ug
Lane 2: NRK Whole Cell Lysate at 40ug
Lane 3: RH35 Whole Cell Lysate at 40ug
Lane 4: Rat Brain Tissue Lysate at 40ug
Lane 5: Rat Liver Tissue Lysate at 40ug
Lane 6: Rat Spleen Tissue Lysate at 40ug
Lane 7: Rat Testis Tissue Lysate at 40ug
Predicted bind size: 23KD
Observed bind size: 23KD
All lanes: Anti-CNTF(ASA-B0465) at 0.5ug/ml
Lane 1: PC-12 Whole Cell Lysate at 40ug
Lane 2: NRK Whole Cell Lysate at 40ug
Lane 3: RH35 Whole Cell Lysate at 40ug
Lane 4: Rat Brain Tissue Lysate at 40ug
Lane 5: Rat Liver Tissue Lysate at 40ug
Lane 6: Rat Spleen Tissue Lysate at 40ug
Lane 7: Rat Testis Tissue Lysate at 40ug
Predicted bind size: 23KD
Observed bind size: 23KD
