Collagen, Type III Antibody (monoclonal)

Collagen, Type III Antibody (monoclonal)

$330.00

In stock

SKU: ASA-B0477 Category:

Description

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
collagen, type III, alpha 1 Monoclonal IgG Mouse Human, Rat IHC-F, WB Ascites

Immunogen
Human collagen type III.

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request.

Concentration

 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml.

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 COL3A1

Protein

 Collagen alpha-1(III) chain

Uniprot ID

 Human(P02461), Mouse(P08121), Rat(P13941)

Function

 Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of GPR56 in the developing brain and binding to GPR56 inhibits neuronal migration and activates the RhoA pathway by coupling GPR56 to GNA13 and possibly GNA12.

Tissue Specificity

Sub-cellular localization

 Secreted, extracellular space, extracellular matrix.

Sequence Similarities

 Belongs to the fibrillar collagen family.

Aliases

 EDS4A|COLLAGEN, FETAL|COLLAGEN, BLOOD VESSEL

Application Details

Application Concentration* Species Validated Using**
Western blot 0.25-0.5μg/ml Human, rat AssaySolutio’s ECL kit
Immunohistochemistry(Frozen Section) 0.5-1μg/ml Human, rat AssaySolutio’s IHC/ICC Detection kit

AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information

Anti-Collagen, Type III antibody (monoclonal), ASA-B0477, Western blotting
Lane 1: HT1080 Cell Lysate
Lane 2: COLO320 Cell Lysate

Anti-Collagen, Type III antibody (monoclonal), ASA-B0477, IHC(F)
IHC(F): Mouse Kidney Tissue

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