Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| heat shock 10kDa protein 1(chaperonin 10) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Cpn10(81-102aa VVLDDKDYFLFRDGDILGKYVD), different from the related rat and mouse sequences by one amino acid. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
HSPE1 |
Protein |
10 kDa heat shock protein, mitochondrial(Hsp10) |
Uniprot ID |
P61604 |
Function |
Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter. |
Tissue Specificity |
|
Sub-cellular localization |
Mitochondrion matrix. |
Sequence Similarities |
|
Aliases |
10 kDa chaperonin antibody|10 kDa heat shock protein mitochondrial antibody|10 kDa heat shock protein, mitochondrial antibody|CH10_HUMAN antibody|Chaperonin 10 antibody|Cpn 10 antibody|CPN10 antibody|Early pregnancy factor antibody|Early-pregnancy factor antibody|EPF antibody|GROES antibody|Heat shock 10kD protein 1 antibody|Heat shock 10kD protein 1 chaperonin 10 antibody|Heat shock 10kDa protein 1 antibody|Heat shock 10kDa protein 1 chaperonin 10 antibody|HSP 10 antibody|HSP10 antibody|HSPE 1 antibody|HSPE1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat Mouse | AssaySolutio’s IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Cpn10 antibody, ASA-B0495, ICC
ICC: A549 Cell
ICC: A549 Cell
Anti-Cpn10 antibody, ASA-B0495, Western blotting
All lanes: Anti Cpn10 (ASA-B0495) at 0.5ug/ml
Lane 1: Rat Thymus Tissue Lysate at 50ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Lane 3: Rat Ovary Tissue Lysate at 50ug
Lane 4: Rat Testis Tissue Lysate at 50ug
Lane 5: A431 Whole Cell Lysate at 40ug
Lane 6: A549 Whole Cell Lysate at 40ug
Lane 7: MCF-7 Whole Cell Lysate at 40ug
Lane 8: MM231 Whole Cell Lysate at 40ug
Lane 9: HELA Whole Cell Lysate at 40ug
Pre
All lanes: Anti Cpn10 (ASA-B0495) at 0.5ug/ml
Lane 1: Rat Thymus Tissue Lysate at 50ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Lane 3: Rat Ovary Tissue Lysate at 50ug
Lane 4: Rat Testis Tissue Lysate at 50ug
Lane 5: A431 Whole Cell Lysate at 40ug
Lane 6: A549 Whole Cell Lysate at 40ug
Lane 7: MCF-7 Whole Cell Lysate at 40ug
Lane 8: MM231 Whole Cell Lysate at 40ug
Lane 9: HELA Whole Cell Lysate at 40ug
Pre
Anti-Cpn10 antibody, ASA-B0495, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue
