Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| cytochrome P450, family 11, subfamily A, polypeptide 1 | Polyclonal | IgG | Rabbit | Human | IHC-P, IHC-F, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human CYP11A1(507-521aa ISFTFWPFNQEATQQ). | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
CYP11A1 |
Protein |
Cholesterol side-chain cleavage enzyme, mitochondrial |
Uniprot ID |
P05108 |
Function |
Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone. |
Tissue Specificity |
|
Sub-cellular localization |
Mitochondrion membrane. |
Sequence Similarities |
|
Aliases |
Cholesterol 20 22 desmolase antibody|Cholesterol desmolase antibody|Cholesterol monooxygenase(side chain cleaving) antibody|Cholesterol side chain cleavage enzyme antibody|Cholesterol side chain cleavage enzyme mitochondrial antibody|Cholesterol side-chain cleavage enzyme antibody|CP11A_HUMAN antibody|CYP 11A antibody|CYP11A antibody|CYP11A1 antibody|CYPXIA1 antibody|Cytochrome P450 11A1 antibody|Cytochrome P450 11A1 mitochondrial antibody|Cytochrome P450 family 11 subfamily A polypeptide 1 antibody|Cytochrome P450 subfamily XIA(cholesterol side chain cleavage) antibody|Cytochrome P450 subfamily XIA antibody|Cytochrome P450(scc) antibody|Cytochrome P450C11A1 antibody|mitochondrial antibody|P450(scc) antibody|P450SCC antibody|Steroid 20 22 lyase antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio’s IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Human | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CYP11A1 antibody, ASA-B0542, Western blotting
WB: Human Placenta Tissue Lysate
WB: Human Placenta Tissue Lysate
Anti-CYP11A1 antibody, ASA-B0542, IHC(P)
IHC(P): Human Placenta Tissue
IHC(P): Human Placenta Tissue
Anti-CYP11A1 antibody, ASA-B0542, IHC(F)
IHC(F): Human Placenta Tissue
IHC(F): Human Placenta Tissue
