Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| cytochrome P450, family 24, subfamily A, polypeptide 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human CYP24A1 recombinant protein (Position: E153-R514). Human CYP24A1 shares 86.2% amino acid (aa) sequence identity with both mouse and rat CYP24A1. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
CYP24A1 |
Protein |
1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial |
Uniprot ID |
Q07973 |
Function |
Has a role in maintaining calcium homeostasis. Catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25- hydroxyvitamin D(3)) and calcitriol (1-alpha,25-dihydroxyvitamin D(3)). The enzyme can perform up to 6 rounds of hydroxylation of calcitriol leading to calcitroic acid. It also shows 23- hydroxylating activity leading to 1-alpha,25-dihydroxyvitamin D(3)-26,23-lactone as end product. |
Tissue Specificity |
|
Sub-cellular localization |
Mitochondrion. |
Sequence Similarities |
|
Aliases |
1 25 @dihydroxyvitamin D3 24 hydroxylase antibody|1 25 dihydroxyvitamin D(3) 24 hydroxylase antibody|1 antibody|1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial antibody|24 ohase antibody|24-OHase antibody|25-dihydroxyvitamin D(3) 24-hydroxylase antibody|CP 24 antibody|CP24 antibody| CP24A_HUMAN antibody|CYP 24 antibody|CYP24 antibody|CYP24A1 antibody| Cytochrome P450 24A1 antibody| Cytochrome P450 24A1 mitochondrial antibody|cytochrome P450 CC24 antibody|Cytochrome P450 family 24 antibody|Cytochrome P450 family 24 subfamily A polypeptide 1 antibody| Cytochrome P450 subfamily XXIV antibody|Cytochrome P450, subfamily XXIV (vitamin D 24-hydroxylase) antibody|Cytochrome P450-CC24 antibody|EC 1.14.13.n4 antibody|Exo mitochondrial protein antibody|HCAI antibody| MGC126273 antibody| MGC126274 antibody|mitochondrial antibody|P450 CC24 antibody|Vitamin D 24 hydroxylase antibody|Vitamin D(3) 24 hydroxylase antibody| Vitamin D(3) 24-hydroxylase antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- CYP24A1 antibody, ASA-B0547, Western blotting
All lanes: Anti CYP24A1 (ASA-B0547) at 0.5ug/ml
Lane 1: Rat Thymus Tissue Lysate at 50ug
Lane 2: Rat Lung Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: 22RV1 Whole Cell Lysate at 40ug
Lane 5: A431 Whole Cell Lysate at 40ug
Lane 6: HEPA Whole Cell Lysate at 40ug
Predicted bind size: 59KD
Observed bind size: 59KD
All lanes: Anti CYP24A1 (ASA-B0547) at 0.5ug/ml
Lane 1: Rat Thymus Tissue Lysate at 50ug
Lane 2: Rat Lung Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: 22RV1 Whole Cell Lysate at 40ug
Lane 5: A431 Whole Cell Lysate at 40ug
Lane 6: HEPA Whole Cell Lysate at 40ug
Predicted bind size: 59KD
Observed bind size: 59KD
Anti- CYP24A1 antibody, ASA-B0547, IHC(P)
IHC(P): Rat Kidney Tissue
IHC(P): Rat Kidney Tissue
Anti- CYP24A1 antibody, ASA-B0547, IHC(P)
IHC(P): Mouse Kidney Tissue
IHC(P): Mouse Kidney Tissue
