Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| cytochrome P450, family 2, subfamily D, polypeptide 6 | Polyclonal | IgG | Rabbit | Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence in the middle region of mouse Cytochrome P450 2D6(194-214aa FACRFEYNDPRFIRLLDLLKD), identical to the related rat sequence. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
CYP2D6 |
Protein |
Cytochrome P450 2D6 |
Uniprot ID |
P10635 |
Function |
Responsible for the metabolism of many drugs and environmental chemicals that it oxidizes. It is involved in the metabolism of drugs such as antiarrhythmics, adrenoceptor antagonists, and tricyclic antidepressants. |
Tissue Specificity |
|
Sub-cellular localization |
Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. |
Sequence Similarities |
Belongs to the cytochrome P450 family. |
Aliases |
CPD6 antibody|CYP2D antibody|CYP2D6 antibody|CYP2D7AP antibody|CYP2D7BP antibody|CYP2D7P2 antibody|CYP2D8P2 antibody|CYP2DL1 antibody|CYPIID6 antibody|Cytochrome P450 DB1 antibody|Cytochrome P450 family 2 subfamily D polypeptide 6 antibody|Debrisoquine 4 hydroxylase antibody|EC 1.14.14.1 antibody|Flavoprotein linked monooxygenase antibody|MGC120389 antibody|MGC120390 antibody|Microsomal monooxygenase antibody|P450 DB1 antibody|P450C2D antibody|P450DB1 antibody|Xenobiotic monooxygenase antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat Mouse | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Rat Mouse | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Cytochrome P450 2D6 antibody, ASA-B0562, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
Lane 3: Rat Brain Tissue Lysate
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
Lane 3: Rat Brain Tissue Lysate
