Cytokeratin Peptide 18 Antibody (monoclonal)

Cytokeratin Peptide 18 Antibody (monoclonal)

$330.00

In stock

SKU: ASA-B0570 Category:

Description

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
keratin 18 Monoclonal IgG Mouse Human, Rat IHC-P, IHC-F, WB Ascites

Immunogen
The human epidermal carcinoma A-431 and MCF-7 human breast cancer cell lines.

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request.

Concentration

 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml.

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 KRT18

Protein

 Keratin, type I cytoskeletal 18

Uniprot ID

 Human(P05783), Mouse(P05784), Rat(Q5BJY9)

Function

 Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

Tissue Specificity

Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.

Sub-cellular localization

 Cytoplasm, perinuclear region. Nucleus, nucleolus.

Sequence Similarities

 Belongs to the intermediate filament family.

Aliases

 K18|Keratin-18|CK-18|Cytokeratin-18|CYK18|Cell proliferation-inducing gene 46 protein

Application Details

Application Concentration* Species Validated Using**
Western blot 0.5-1μg/ml Human, rat AssaySolutio’s ECL kit
Immunohistochemistry(Paraffin-embedded Section) 1-2μg/ml Human, rat AssaySolutio’s IHC/ICC Detection kit
Immunohistochemistry(Frozen Section) 0.5-2μg/ml Human, rat AssaySolutio’s IHC/ICC Detection kit

AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information

Anti-Cytokeratin Peptide 18 antibody (monoclonal), ASA-B0570, Western blotting
WB: MCF-7 Cell Lysate
Anti-Cytokeratin Peptide 18 antibody (monoclonal), ASA-B0570, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Anti-Cytokeratin Peptide 18 antibody (monoclonal), ASA-B0570, IHC(P)
IHC(P): Human Rectal Cancer Tissue

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