Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| DEAD(Asp-Glu-Ala-Asp) box polypeptide 4 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, IHC-F, ICC, WB | Immunogen affinity purified. |
Immunogen |
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| A synthetic peptide corresponding to a sequence in the middle region of human DDX4(253-272aa EDEDSIFAHYQTGINFDKYD), identical to the the related rat and mouse sequences. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
DDX4 |
Protein |
Probable ATP-dependent RNA helicase DDX4 |
Uniprot ID |
Q9NQI0 |
Function |
May play a role in germ cell development. May play a role in sperm motility. |
Tissue Specificity |
Expressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes. |
Sub-cellular localization |
Cytoplasm. |
Sequence Similarities |
Belongs to the DEAD box helicase family. DDX4/VASA subfamily. |
Aliases |
DDX 4 antibody|Ddx4 antibody|DDX4_HUMAN antibody|DEAD(Asp Glu Ala Asp) box polypeptide 4 antibody|DEAD box protein 4 antibody|Dead-box protein 4 antibody|DEAD/H(Asp-Glu-Ala-Asp/His) box polypeptide 4 antibody|MGC111074 antibody|MVH antibody|Probable ATP dependent RNA helicase DDX4 antibody|Probable ATP-dependent RNA helicase DDX4 antibody|VASA antibody|VASA homolog antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio’s IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Rat Mouse | AssaySolutio’s IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-DDX4/MVH antibody, ASA-B0580, Western blotting
Lane 1: Rat Testis Tissue Lysate
Lane 2: Mouse Testis Tissue Lysate
Lane 3: HELA Cell Lysate
Lane 1: Rat Testis Tissue Lysate
Lane 2: Mouse Testis Tissue Lysate
Lane 3: HELA Cell Lysate
Anti-DDX4/MVH antibody, ASA-B0580, Western blotting
Lane 1: Rat Testis Tissue Lysate
Lane 2: Mouse Testis Tissue Lysate
Lane 3: Mouse Ovary Tissue Lysate
Lane 1: Rat Testis Tissue Lysate
Lane 2: Mouse Testis Tissue Lysate
Lane 3: Mouse Ovary Tissue Lysate
Anti-DDX4/MVH antibody, ASA-B0580, IHC(P)
IHC(P): Rat Ovary Tissue
IHC(P): Rat Ovary Tissue
