Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| desmin | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, IHC-F, WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human Desmin recombinant protein (Position: M1-T304). Human Desmin shares 97% amino acid (aa) sequence identity with both mouse and rat Desmin. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
DES |
Protein |
Desmin |
Uniprot ID |
P17661 |
Function |
Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures. |
Tissue Specificity |
|
Sub-cellular localization |
Cytoplasm. Cell membrane, sarcolemma. |
Sequence Similarities |
Belongs to the intermediate filament family. |
Aliases |
CMD1I antibody|CSM1 antibody|CSM2 antibody|DES antibody|DESM_HUMAN antibody|Desmin antibody|FLJ12025 antibody|FLJ39719 antibody|FLJ41013 antibody|FLJ41793 antibody|Intermediate filament protein antibody|OTTHUMP00000064865 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio’s IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Mouse, Rat | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Desmin antibody, ASA-B0586–1.jpg
All lanes: Anti Desmin (ASA-B0586) at 0.5ug/ml
WB: Recombinant Human Desmin Protein 0.5ng
Predicted bind size: 36KD
Observed bind size: 36KD
All lanes: Anti Desmin (ASA-B0586) at 0.5ug/ml
WB: Recombinant Human Desmin Protein 0.5ng
Predicted bind size: 36KD
Observed bind size: 36KD
Anti-Desmin antibody, ASA-B0586–2.jpg
All lanes: Anti Desmin (ASA-B0586) at 0.5ug/ml
Lane 1: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 2: Rat Cardiac MuscleTissue Lysate at 50ug
Lane 3: Mouse Skeletal Muscle Tissue Lysate at 50ug
Lane 4: Mouse Cardiac MuscleTissue Lysate at 50ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: HT1080 Whole Cell Lysate at 40ug
Lane 7: COLO320 Whole Cell Lysate at 40ug
Lane 8: HEPA Whole Cell Lysate at 40ug
Lane
All lanes: Anti Desmin (ASA-B0586) at 0.5ug/ml
Lane 1: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 2: Rat Cardiac MuscleTissue Lysate at 50ug
Lane 3: Mouse Skeletal Muscle Tissue Lysate at 50ug
Lane 4: Mouse Cardiac MuscleTissue Lysate at 50ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: HT1080 Whole Cell Lysate at 40ug
Lane 7: COLO320 Whole Cell Lysate at 40ug
Lane 8: HEPA Whole Cell Lysate at 40ug
Lane
Anti-Desmin antibody, ASA-B0586–3.jpg
IHC(F): Rat Cardiac Muscle Tissue
IHC(F): Rat Cardiac Muscle Tissue
