Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| dopamine receptor D2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Dopamine D2 Receptor(360-375aa RRKLSQQKEKKATQML), identical to the related rat and mouse sequences. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
DRD2 |
Protein |
D(2) dopamine receptor |
Uniprot ID |
P14416 |
Function |
Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase. |
Tissue Specificity |
|
Sub-cellular localization |
Cell membrane. |
Sequence Similarities |
|
Aliases |
D(2) dopamine receptor antibody|D2 dopamine receptor antibody|D2DR antibody|D2R antibody|Dopamine D2 receptor antibody|Dopamine receptor D2 antibody|DRD 2 antibody|DRD2 antibody|DRD2_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Dopamine D2 Receptor antibody, ASA-B0602, Western blotting
All lanes: Anti Dopamine D2 Receptor (ASA-B0602) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: SKOV Whole Cell Lysate at 40ug
Lane 3: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 51KD
Observed bind size: 70KD
All lanes: Anti Dopamine D2 Receptor (ASA-B0602) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: SKOV Whole Cell Lysate at 40ug
Lane 3: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 51KD
Observed bind size: 70KD
Anti-Dopamine D2 Receptor antibody, ASA-B0602, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue
