Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| dopamine receptor D3 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Dopamine Receptor D3(352-367aa NTHCQTCHVSPELYSA), different from the related rat and mouse sequences by two amino acids. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
DRD3 |
Protein |
D(3) dopamine receptor |
Uniprot ID |
P35462 |
Function |
Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase. Promotes cell proliferation. |
Tissue Specificity |
Brain. |
Sub-cellular localization |
Cell membrane. |
Sequence Similarities |
Belongs to the G-protein coupled receptor 1 family. |
Aliases |
3dopamine receptor antibody|D antibody|D(3) dopamine receptor antibody|Dopamine D3 receptor antibody|Dopamine receptor D3 antibody|DRD3 antibody|DRD3_HUMAN antibody|ETM1 antibody|FET1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Rat Human, Mouse | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Dopamine Receptor D3 antibody, ASA-B0605, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue
Anti-Dopamine Receptor D3 antibody, ASA-B0605, Western blotting
Lane 1: Rat Testis Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: U87 Cell Lysate
Lane 4: HELA Cell Lysate
Lane 1: Rat Testis Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: U87 Cell Lysate
Lane 4: HELA Cell Lysate
