Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| deoxyuridine triphosphatase | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human DUT(212-229aa KKGDRIAQLICERIFYPE), different from the related rat and mouse sequences by two amino acids. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
DUT |
Protein |
Deoxyuridine 5′-triphosphate nucleotidohydrolase, mitochondrial |
Uniprot ID |
P33316 |
Function |
This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA. |
Tissue Specificity |
Found in a variety of tissues. Isoform 3 expression is constitutive, while isoform 2 expression correlates with the onset of DNA replication (at protein level). Isoform 2 degradation coincides with the cessation of nuclear DNA replication (at protein level). |
Sub-cellular localization |
Isoform 2: Nucleus. |
Sequence Similarities |
Belongs to the dUTPase family. |
Aliases |
Deoxyuridine 5′ triphosphate nucleotidohydrolase, nuclear isoform antibody|Deoxyuridine 5′-triphosphate nucleotidohydrolase, mitochondrial antibody|Deoxyuridine triphosphatase antibody|dut antibody|DUT_HUMAN antibody|dUTP nucleotidohydrolase antibody|dUTP pyrophosphatase antibody|dUTPase antibody|FLJ20622 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-DUT antibody, ASA-B0613, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue
Anti-DUT antibody, ASA-B0613, Western blotting
All lanes: Anti DUT (ASA-B0613) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 27KD
Observed bind size: 27KD
All lanes: Anti DUT (ASA-B0613) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 27KD
Observed bind size: 27KD
