Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| fatty acid binding protein 6, ileal | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human FABP6(15-33aa YDEFMKLLGISSDVIEKAR). | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
FABP6 |
Protein |
Gastrotropin |
Uniprot ID |
P51161 |
Function |
Ileal protein which stimulates gastric acid and pepsinogen secretion. Seems to be able to bind to bile salts and bilirubins. Isoform 2 is essential for the survival of colon cancer cells to bile acid-induced apoptosis. |
Tissue Specificity |
Isoform 2 is expressed in colorectal adenocarcinomas and their adjacent normal mucosa (at protein level). Isoform 1 is expressed in the jejunum, ileum, cecum and ascending colon intestine. Isoform 2 is expressed in the gallbladder, duodenum, jejunum, ileum, cecum, ascending, transverse and descending colon, sigmoid colon and rectum. |
Sub-cellular localization |
Isoform 1: Cytoplasm. |
Sequence Similarities |
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. |
Aliases |
Fabp6 antibody|FABP6_HUMAN antibody|Fatty acid binding protein 6, ileal(gastrotropin) antibody|Fatty acid-binding protein 6 antibody|Gastrotropin antibody|GT antibody|I 15P antibody|I BABP antibody|I BALB antibody|I BAP antibody|I-15P antibody|I-BABP antibody|I15P antibody|IBABP antibody|IBALB antibody|IBAP antibody|ILBP antibody|ILBP3 antibody|Ileal lipid binding protein antibody|Ileal lipid-binding protein antibody|ILLBP antibody|Intestinal 15 kDa protein antibody|Intestinal bile acid binding protein antibody|Intestinal bile acid-binding protein antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-FABP6 antibody, ASA-B0676, IHC(P)
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue
Anti-FABP6 antibody, ASA-B0676, Western blotting
All lanes: Anti FABP6 (ASA-B0676) at 0.5ug/ml
Lane 1: COLO320 Whole Cell Lysate at 40ug
Lane 2: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 14KD
Observed bind size: 14KD
All lanes: Anti FABP6 (ASA-B0676) at 0.5ug/ml
Lane 1: COLO320 Whole Cell Lysate at 40ug
Lane 2: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 14KD
Observed bind size: 14KD
