Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| fibroblast growth factor 19 | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence in the middle region of human FGF19(124-140aa EEIRPDGYNVYRSEKHR). | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
FGF19 |
Protein |
Fibroblast growth factor 19(FGF-19) |
Uniprot ID |
O95750 |
Function |
Involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression, following positive regulation of the JNK and ERK1/2 cascades. Stimulates glucose uptake in adipocytes. Activity requires the presence of KLB and FGFR4. |
Tissue Specificity |
Expressed in fetal brain, cartilage, retina, and adult gall bladder. |
Sub-cellular localization |
Secreted. |
Sequence Similarities |
|
Aliases |
FGF 19 antibody|FGF-19 antibody|FGF15 antibody|FGF19 antibody|FGF19_HUMAN antibody|Fibroblast growth factor 15 antibody|Fibroblast growth factor 19 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-FGF19 antibody, ASA-B0700, Western blotting
All lanes: Anti FGF19 (ASA-B0700) at 0.5ug/ml
Lane 1: U87 Whole Cell Lysate at 40ug
Lane 2: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 24KD
Observed bind size: 24KD
All lanes: Anti FGF19 (ASA-B0700) at 0.5ug/ml
Lane 1: U87 Whole Cell Lysate at 40ug
Lane 2: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 24KD
Observed bind size: 24KD
Anti-FGF19 antibody, ASA-B0700, IHC(P)
IHC(P): Human Gallbladder Cancer Tissue
IHC(P): Human Gallbladder Cancer Tissue
