Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| glutamate decarboxylase 2(pancreatic islets and brain, 65kDa) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human GAD65(21-37aa ENPGTARAWCQVAQKFT), identical to the related mouse and rat sequences. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
GAD2 |
Protein |
Glutamate decarboxylase 2 |
Uniprot ID |
Q05329 |
Function |
Catalyzes the production of GABA. |
Tissue Specificity |
|
Sub-cellular localization |
Cytoplasm, cytosol. |
Sequence Similarities |
Belongs to the group II decarboxylase family. |
Aliases |
65 kDa glutamic acid decarboxylase antibody|DCE 2 antibody|DCE2 antibody|DCE2_HUMAN antibody|GAD 2 antibody|GAD 65 antibody|GAD-2 antibody|GAD-65 antibody|Gad2 antibody|Glutamate decarboxylase 2(pancreas) antibody|Glutamate Decarboxylase 2(pancreatic islets and brain 65kDa) antibody|Glutamate decarboxylase 2 antibody|Glutamate Decarboxylase 65 antibody|Glutamate decarboxylase 65 kDa isoform antibody|Glutamic Acid Decarboxylase 2 antibody|Glutamic Acid Decarboxylase 65 antibody|MGC161605 antibody|MGC161607 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Rat Human, Mouse | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-GAD65 antibody, ASA-B0749, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Anti-GAD65 antibody, ASA-B0749, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue
