Gelsolin Antibody (monoclonal)

Gelsolin Antibody (monoclonal)

$330.00

In stock

SKU: ASA-B0778 Category:

Description

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
gelsolin Monoclonal IgG Mouse Human, Rabbit IHC-P, IHC-F, WB Ascites

Immunogen
Human plasma gelsolin.

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request.

Concentration

 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml.

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 GSN

Protein

 Gelsolin

Uniprot ID

 Human(P06396), Mouse(P13020), Rat(Q68FP1)

Function

 Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.

Tissue Specificity

Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells.

Sub-cellular localization

 Isoform 2: Cytoplasm, cytoskeleton.

Sequence Similarities

 Belongs to the villin/gelsolin family.

Aliases

 AGEL|ADF|Brevin|Actin-depolymerizing factor

Application Details

Application Concentration* Species Validated Using**
Western blot 0.5-1μg/ml Human, rabbit AssaySolutio’s ECL kit
Immunohistochemistry(Paraffin-embedded Section) 1-2μg/ml Human, rabbit AssaySolutio’s IHC/ICC Detection kit
Immunohistochemistry(Frozen Section) 1-2μg/ml Human, rabbit AssaySolutio’s IHC/ICC Detection kit

AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information

Anti- Gelsolin antibody, ASA-B0778, IHC(P)
IHC(P): Human Lung Cancer Tissue
Anti- Gelsolin antibody, ASA-B0778, IHC(P)
IHC(P): Human Intestinal Cancer Tissue

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