Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| solute carrier family 2(facilitated glucose transporter), member 4 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Glucose Transporter GLUT4(491-509aa EQEVKPSTELEYLGPDEND), identical to the related rat and mouse sequences. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
SLC2A4 |
Protein |
Solute carrier family 2, facilitated glucose transporter member 4 |
Uniprot ID |
P14672 |
Function |
Insulin-regulated facilitative glucose transporter. |
Tissue Specificity |
Skeletal and cardiac muscles; brown and white fat. |
Sub-cellular localization |
Cell membrane ; Multi-pass membrane protein . Endomembrane system. |
Sequence Similarities |
Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily. |
Aliases |
insulin-responsive antibody|Glucose transporter GLUT 4 antibody|Glucose transporter type 4 antibody|Glucose transporter type 4 insulin responsive antibody|GLUT 4 antibody|GLUT-4 antibody|GLUT4 antibody|GTR4_HUMAN antibody|kug antibody|SLC 2A4 antibody|SLC2A4 antibody|solute carrier family 2(facilitated glucose transporter) member 4 antibody|Solute carrier family 2, facilitated glucose transporter member 4 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Glucose Transporter GLUT4 antibody, ASA-B0796, IHC(P)
IHC(P): Rat Cardiac Muscle Tissue
IHC(P): Rat Cardiac Muscle Tissue
Anti-Glucose Transporter GLUT4 antibody, ASA-B0796, Western blotting
All lanes: Anti Glucose Transporter GLUT4 (ASA-B0796) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 2: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 3: Mouse Cardiac Muscle Tissue Lysate at 50ug
Lane 4: Mouse Skeletal Muscle Tissue Lysate at 50ug
Lane 5: HELA Whole Cell Lysate at 40ug
Predicted bind size: 55 KD
Observed bind size: 55 KD
All lanes: Anti Glucose Transporter GLUT4 (ASA-B0796) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 2: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 3: Mouse Cardiac Muscle Tissue Lysate at 50ug
Lane 4: Mouse Skeletal Muscle Tissue Lysate at 50ug
Lane 5: HELA Whole Cell Lysate at 40ug
Predicted bind size: 55 KD
Observed bind size: 55 KD
