Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| glutathione peroxidase 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence in the middle region of human GPX1(116-146aa EVNGAGAHPLFAFLREALPAPSDDATALMTD), different from the related mouse sequence by six amino acids and from the related rat sequence by five amino acids. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
GPX1 |
Protein |
Glutathione peroxidase 1 |
Uniprot ID |
P07203 |
Function |
Protects the hemoglobin in erythrocytes from oxidative breakdown. |
Tissue Specificity |
|
Sub-cellular localization |
Cytoplasm. |
Sequence Similarities |
Belongs to the glutathione peroxidase family. |
Aliases |
AL033363 antibody|Cellular glutathione peroxidase antibody|Glutathione peroxidase 1 antibody|Glutathione peroxidase antibody|GPx 1 antibody|GPx-1 antibody|GPX1 antibody|GPX1_HUMAN antibody|GPXD antibody|GSHPx-1 antibody|GSHPX1 antibody|MGC14399 antibody|MGC88245 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- GPX1 antibody, ASA-B0808, Western blotting
All lanes: Anti GPX1 (ASA-B0808) at 0.5ug/ml
WB: Recombinant Human GPX1 Protein 0.5ng
Predicted bind size: 36KD
Observed bind size: 36KD
All lanes: Anti GPX1 (ASA-B0808) at 0.5ug/ml
WB: Recombinant Human GPX1 Protein 0.5ng
Predicted bind size: 36KD
Observed bind size: 36KD
Anti- GPX1 antibody, ASA-B0808, Western blotting
All lanes: Anti GPX1 (ASA-B0808) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 50ug
Lane 2: Mouse Kidney Tissue Lysate at 50ug
Lane 3: Rat Spleen Tissue Lysate at 50ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 22,44KD
Observed bind size: 22,44KD
All lanes: Anti GPX1 (ASA-B0808) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 50ug
Lane 2: Mouse Kidney Tissue Lysate at 50ug
Lane 3: Rat Spleen Tissue Lysate at 50ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 22,44KD
Observed bind size: 22,44KD
Anti- GPX1 antibody, ASA-B0808, IHC(P)
IHC(P): Mosue Pancreas Tissue
IHC(P): Mosue Pancreas Tissue
