Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| glutathione S-transferase pi 1 | Polyclonal | IgG | Rabbit | Human, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human GST3 recombinant protein (Position: P2-Q210). Human GST3 shares 85% and 86% amino acid (aa) sequences identity with mouse and rat GST3, respectively. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
GSTP1 |
Protein |
Glutathione S-transferase P |
Uniprot ID |
P09211 |
Function |
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration. |
Tissue Specificity |
|
Sub-cellular localization |
Cytoplasm. |
Sequence Similarities |
Belongs to the GST superfamily. Pi family. |
Aliases |
Deafness antibody|Deafness X-linked 7 antibody|DFN7 antibody|FAEES3 antibody|Fatty Acid Ethyl Ester Synthase III antibody|Glutathione S Transferase 3 antibody|Glutathione S Transferase Pi antibody|Glutathione S-transferase P antibody|Glutathione S-transferase pi 1 antibody|GST class-pi antibody|GST3 antibody|GSTP antibody|Gstp1 antibody|GSTP1-1 antibody|GSTP1_HUMAN antibody|PI antibody|X linked 7 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- GST3/GST pi antibody, ASA-B0831, Western blotting
All lanes: Anti GST3/GST pi(ASA-B0831) at 0.5ug/ml
WB: Recombinant Human GST3 Protein 0.5ng
Predicted bind size: 32KD
Observed bind size: 32KD
All lanes: Anti GST3/GST pi(ASA-B0831) at 0.5ug/ml
WB: Recombinant Human GST3 Protein 0.5ng
Predicted bind size: 32KD
Observed bind size: 32KD
Anti- GST3/GST pi antibody, ASA-B0831, Western blotting
All lanes: Anti GST3/GST pi (ASA-B0831) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Lane 3: Rat Liver Tissue Lysate at 50ug
Lane 4: Hela Whole Cell Lysate at 40ug
Lane 5: HT1080 Whole Cell Lysate at 40ug
Lane 6: MCF-7 Whole Cell Lysate at 40ug
Lane 7: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 23KD
Observed bind size: 23KD
All lanes: Anti GST3/GST pi (ASA-B0831) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Lane 3: Rat Liver Tissue Lysate at 50ug
Lane 4: Hela Whole Cell Lysate at 40ug
Lane 5: HT1080 Whole Cell Lysate at 40ug
Lane 6: MCF-7 Whole Cell Lysate at 40ug
Lane 7: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 23KD
Observed bind size: 23KD
Anti- GST3/GST pi antibody, ASA-B0831, IHC(P)
IHC(P): Rat Lung Tissue
IHC(P): Rat Lung Tissue
