Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| major histocompatibility complex, class II, DM beta | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence in the middle region of human HLA DMB(81-100aa NQKDTLMQRLRNGLQNCATH). | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
HLA-DMB |
Protein |
HLA class II histocompatibility antigen, DM beta chain |
Uniprot ID |
P28068 |
Function |
Plays a critical role in catalyzing the release of class II-associated invariant chain peptide (CLIP) from newly synthesized MHC class II molecules and freeing the peptide binding site for acquisition of antigenic peptides. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. |
Tissue Specificity |
|
Sub-cellular localization |
Late endosome membrane. |
Sequence Similarities |
Belongs to the MHC class II family. |
Aliases |
class II antigen HLA-DM beta chain antibody|class II histocompatibility antigen antibody|Class II histocompatibility antigen M beta chain antibody|D6S221E antibody|DMB antibody|HLA class II histocompatibility antigen DM beta chain antibody|HLA-DM histocompatibility type, beta chain antibody|M beta chain antibody|Major histocompatibility complex class II DM beta antibody|MHC class II antigen DMB antibody|MHC class II antigen HLA DM beta chain antibody|MHC class II HLA DMB antibody|OTTHUMP00000029257 antibody|Really interesting new gene 7 protein antibody|RING 7 antibody|RING7 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-HLA DMB antibody, ASA-B0873, Western blotting
All lanes: Anti HLA DMB (ASA-B0873) at 0.5ug/ml
Lane 1: JURKAT Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Lane 3: RAJI Whole Cell Lysate at 40ug
Lane 4: HUT Whole Cell Lysate at 40ug
Predicted bind size: 29KD
Observed bind size: 29KD
All lanes: Anti HLA DMB (ASA-B0873) at 0.5ug/ml
Lane 1: JURKAT Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Lane 3: RAJI Whole Cell Lysate at 40ug
Lane 4: HUT Whole Cell Lysate at 40ug
Predicted bind size: 29KD
Observed bind size: 29KD
Anti-HLA DMB antibody, ASA-B0873, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
