Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| major histocompatibility complex, class I, C | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human HLA-C(38-62aa EYWDRETQKYKRQAQADRVNLRKLR). | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
HLA-C |
Protein |
MHC class I antigen |
Uniprot ID |
U5YBW8 |
Function |
|
Tissue Specificity |
|
Sub-cellular localization |
|
Sequence Similarities |
|
Aliases |
1C07_HUMAN antibody|Cw-7 alpha chain antibody|D6S204 antibody|HLA class I histocompatibility antigen antibody|HLA class I histocompatibility antigen, C alpha chain antibody|HLA class I histocompatibility antigen, Cw-1 alpha chain antibody| HLA-C antibodyHLA-C histocompatibility type antibody|HLA-Cw antibody|HLA-JY3 antibody|HLAC antibody|HLC-C antibody|human leukocyte antigen-C alpha chain antibody|major histocompatibility antigen HLA-C antibody|major histocompatibility complex, class I, C antibody|MHC class I antigen antibody|MHC class I antigen Cw 1 antibody|MHC class I antigen Cw*7 antibody|MHC class I antigen heavy chain HLA-C antibody|PSORS1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio’s IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- HLA-C antibody, ASA-B0872, Western blotting
All lanes: Anti HLA-C (ASA-B0872) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: U87 Whole Cell Lysate at 40ug
Lane 4: Rat Brain Tissue Lysate at 50ug
Lane 5: Rat Liver Tissue Lysate at 50ug
Predicted bind size: 41KD
Observed bind size: 41KD
All lanes: Anti HLA-C (ASA-B0872) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: U87 Whole Cell Lysate at 40ug
Lane 4: Rat Brain Tissue Lysate at 50ug
Lane 5: Rat Liver Tissue Lysate at 50ug
Predicted bind size: 41KD
Observed bind size: 41KD
Anti- HLA-C antibody, ASA-B0872,IHC(P)
IHC(P): Mouse Intestine Tissue
IHC(P): Mouse Intestine Tissue
Anti- HLA-C antibody, ASA-B0872,IHC(P)
IHC(P): Rat Intestine Tissue
IHC(P): Rat Intestine Tissue
