Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| carcinoembryonic antigen-related cell adhesion molecule 5 | Polyclonal | IgG | Rabbit | Human | ELISA, IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| Insect cells-derived human CEA recombinant protein(Position: full). | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
CEACAM5 |
Protein |
Carcinoembryonic antigen-related cell adhesion molecule 5 |
Uniprot ID |
P06731 |
Function |
Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins. |
Tissue Specificity |
Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon. |
Sub-cellular localization |
Cell membrane. |
Sequence Similarities |
Belongs to the immunoglobulin superfamily. CEA family. |
Aliases |
Carcinoembryonic antigen antibody|Carcinoembryonic antigen related cell adhesion molecule 5 antibody|Carcinoembryonic antigen-related cell adhesion molecule 5 antibody|CD66e antibody|CD66e antigen antibody|CEA antibody|Ceacam5 antibody|Meconium antigen 100 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio’s IHC/ICC Detection kit |
| ELISA | 0.1-0.5μg/ml | Human | Sandwich ELISA format |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-human CEACAM5 antibody, ASA-B0934, Western blotting
Lane 1: Recombinant Human CEA Protein 5ng
Lane 2: Recombinant Human CEA Protein 2
Lane 1: Recombinant Human CEA Protein 5ng
Lane 2: Recombinant Human CEA Protein 2
