Description
Overview
Long Name
|
Antibody Type
|
Antibody Isotype
|
Host
|
Species Reactivity
|
Validated Applications
|
Purification
|
| D-dopachrome tautomerase |
Polyclonal |
IgG |
Rabbit |
Human |
ELISA, IHC-P, ICC, WB |
Immunogen affinity purified. |
Immunogen
|
| E. coli-derived human DDT recombinant protein(Position: M1-L118). |
Properties
Form
|
Lyophilized |
Size
|
100 µg/vial |
Contents
|
Antibody is lyophilized with 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration
|
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage
|
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene
|
DDT |
Protein
|
D-dopachrome decarboxylase |
Uniprot ID
|
P30046 |
Function
|
Tautomerization of D-dopachrome with decarboxylation to give 5,6-dihydroxyindole (DHI). |
Tissue Specificity
|
|
Sub-cellular localization
|
Cytoplasm . |
Sequence Similarities
|
|
Aliases
|
D dopachrome decarboxylase antibody|D dopachrome tautomerase antibody|D-dopachrome decarboxylase antibody|D-dopachrome tautomerase antibody|DDCT antibody|Ddt antibody|Phenylpyruvate tautomerase II antibody |
Application Details
| Application |
Concentration* |
Species |
Validated Using** |
| Western blot |
0.1-0.5μg/ml |
Human |
AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) |
0.5-1μg/ml |
Human |
AssaySolutio’s IHC/ICC Detection kit |
| Immunocytochemistry |
0.5-1μg/ml |
Human |
AssaySolutio’s IHC/ICC Detection kit |
| ELISA |
0.1-0.5μg/ml |
Human |
Sandwich ELISA format |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
