Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| erythropoietin | Polyclonal | IgG | Rabbit | Human | ELISA, IP, IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| E. coli-derived human EPO recombinant protein(Position: A1-R166). | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
EPO |
Protein |
Erythropoietin |
Uniprot ID |
P01588 |
Function |
Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass. |
Tissue Specificity |
Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals. |
Sub-cellular localization |
Secreted. |
Sequence Similarities |
Belongs to the EPO/TPO family. |
Aliases |
EP antibody|EPO alpha antibody|EPO antibody|Epoetin antibody|Erythropoetin antibody|Erythropoietin antibody|Erythropoietin precursor antibody|MGC138142 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio’s IHC/ICC Detection kit |
| ELISA | 0.1-0.5μg/ml | Human | Sandwich ELISA format |
| Immunoprecipitation | Human | Human |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-human EPO antibody, ASA-B0936, Western blotting
Lane 1: Recombinant human EPO Protein 10ng
Lane 2: Recombinant human EPO Protein 5ng
Lane 3: Recombinant human EPO Protein 2
Lane 1: Recombinant human EPO Protein 10ng
Lane 2: Recombinant human EPO Protein 5ng
Lane 3: Recombinant human EPO Protein 2
Anti-human EPO antibody, ASA-B0936, IHC(P)
IHC(P): Human Placenta Tissue
IHC(P): Human Placenta Tissue
