Description
Overview
Long Name
|
Antibody Type
|
Antibody Isotype
|
Host
|
Species Reactivity
|
Validated Applications
|
Purification
|
| interleukin 10 |
Polyclonal |
IgG |
Rabbit |
Human |
ELISA, IHC-P, WB |
Immunogen affinity purified. |
Immunogen
|
| E. coli-derived human IL-10 recombinant protein(Position: S19-N178). |
Properties
Form
|
Lyophilized |
Size
|
100 µg/vial |
Contents
|
Antibody is lyophilized with 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration
|
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage
|
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene
|
IL10 |
Protein
|
Interleukin-10(IL-10) |
Uniprot ID
|
P22301 |
Function
|
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. |
Tissue Specificity
|
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. |
Sub-cellular localization
|
Secreted. |
Sequence Similarities
|
Belongs to the IL-10 family. |
Aliases
|
CSIF antibody|Cytokine Synthesis Inhibitory Factor antibody|GVHDS antibody|IL 10 antibody|IL-10 antibody|IL10 antibody|IL10A antibody|Interleukin 10 antibody|Interleukin-10 antibody|Interleukin10 antibody|MGC126450 antibody|MGC126451 antibody|T-cell growth inhibitory factor antibody|TGIF antibody |
Application Details
| Application |
Concentration* |
Species |
Validated Using** |
| Western blot |
0.1-0.5μg/ml |
Human |
AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) |
0.5-1μg/ml |
Human |
AssaySolutio’s IHC/ICC Detection kit |
| ELISA |
0.1-0.5μg/ml |
Human |
Sandwich ELISA format |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
