Description
Overview
Long Name
|
Antibody Type
|
Antibody Isotype
|
Host
|
Species Reactivity
|
Validated Applications
|
Purification
|
| interleukin 3(colony-stimulating factor, multiple) |
Polyclonal |
IgG |
Rabbit |
Human |
ELISA, Neu, IP, IHC-P, WB |
Immunogen affinity purified. |
Immunogen
|
| E. coli-derived human IL-3 recombinant protein(Position: A20-F152). |
Properties
Form
|
Lyophilized |
Size
|
100 µg/vial |
Contents
|
Antibody is lyophilized with 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration
|
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage
|
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene
|
IL3 |
Protein
|
Interleukin-3(IL-3) |
Uniprot ID
|
P08700 |
Function
|
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. |
Tissue Specificity
|
Activated T-cells, mast cells, natural killer cells. |
Sub-cellular localization
|
Secreted. |
Sequence Similarities
|
Belongs to the IL-3 family. |
Aliases
|
Burst promoting activity antibody|Colony stimulating factor multiple antibody|Eosinophil colony stimulating factor antibody|Hematopoietic growth factor antibody|IL 3 antibody|IL-3 antibody|IL3 antibody|Interleukin 3 (colony stimulating factor, multiple) antibody|Interleukin 3 antibody|Interleukin-3 antibody|Mast cell growth factor antibody|MCGF antibody|MGC79398 antibody|MGC79399 antibody|Multi CSF antibody|Multilineage colony stimulating factor antibody|Multipotential colony stimulating factor antibody|Multipotential colony-stimulating factor antibody|OTTHUMP00000065963 antibody|P cell stimulating factor antibody|P-cell-stimulating factor antibody|Thy1 inducing factor antibody |
Application Details
| Application |
Concentration* |
Species |
Validated Using** |
| Western blot |
0.1-0.5μg/ml |
Human |
AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) |
0.5-1μg/ml |
Human |
AssaySolutio’s IHC/ICC Detection kit |
| ELISA |
0.1-0.5μg/ml |
Human |
Sandwich ELISA format |
| Neutralization |
Human |
Human |
|
| Immunoprecipitation |
Human |
Human |
|
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
