Description
Overview
Long Name
|
Antibody Type
|
Antibody Isotype
|
Host
|
Species Reactivity
|
Validated Applications
|
Purification
|
| interleukin 4 |
Polyclonal |
IgG |
Rabbit |
Human |
ELISA, Neu, IP, IHC-P, WB |
Immunogen affinity purified. |
Immunogen
|
| E. coli-derived human IL-4 recombinant protein(Position: H25-S153). |
Properties
Form
|
Lyophilized |
Size
|
100 µg/vial |
Contents
|
Antibody is lyophilized with 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration
|
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage
|
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene
|
IL4 |
Protein
|
Interleukin-4(IL-4) |
Uniprot ID
|
P05112 |
Function
|
Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. |
Tissue Specificity
|
|
Sub-cellular localization
|
Secreted. |
Sequence Similarities
|
Belongs to the IL-4/IL-13 family. |
Aliases
|
B cell growth factor 1 antibody|B cell IgG differentiation factor antibody|B Cell Stimulatory Factor 1 antibody|B-cell stimulatory factor 1 antibody|BCGF 1 antibody|BCGF1 antibody|Binetrakin antibody|BSF 1 antibody|BSF-1 antibody|BSF1 antibody|HCGF antibody|Hodgkin |
Application Details
| Application |
Concentration* |
Species |
Validated Using** |
| Western blot |
0.1-0.5μg/ml |
Human |
AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) |
0.5-1μg/ml |
Human |
AssaySolutio’s IHC/ICC Detection kit |
| ELISA |
0.1-0.5μg/ml |
Human |
Sandwich ELISA format |
| Neutralization |
Human |
Human |
|
| Immunoprecipitation |
Human |
Human |
|
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
